Cytochrome P450 (CYP) monooxygenases, the nature’s most versatile biological catalysts have unique ability to catalyse regio-, chemo-, and stereospecific oxidation of a wide range of substrates under mild reaction conditions, thereby addressing a significant challenge in chemocatalysis. In the recent decades, the importance of CYPs is illustrated by the fact that they are responsible for the majority of Phase I reactions in human drug metabolism, and their substrates include a broad range of active pharmaceutical ingredients, environmental toxins, carcinogens, and food-derived chemicals. Therefore, a compilation of CYP monooxygenases is required for a rational comprehensive approach for elucidating the catalytic potentials and functional utilities of human CYPs for industrial approach. This urged us to generate the gene library of human cytochrome P450s to facilitate the glimpse of molecular mechanisms and metabolic diversity of P450s, thereby functionally explore and characterize the novel enzymes.  Here I have listed the three major ongoing projects which I am currently engaged in Prof. Bureik's laboratory. 

1.      Construction of Human CYP Gene Library

2.      Functional Expression of Human CYPs in Fission Yeast Reconstituted System

3.      Systematic assessment of CYP-dependent metabolism with Differential Redox partners