Ruibing Chen——Professor
Nationality:
Chinese
Phone:
Email:
rbchen@tju.edu.cn
Office:
Room 314-7, Building #24
School:
School of Pharmaceutical Science and Technology
ResearcherID:
Group weblink
Education Experience
2001-2005 Bachelor Applied Chemistry Peking University
2005-2009 Ph. D. Analytical Chemistry University of Wisconsin-Madison
Professional Experience
2010-2016 Associate Professor Tianjin Medical University
2016-2019 Professor Tianjin Medical University
2019-2019 Professor Tianjin University
Research Area

Our research is focused on developing and implementing proteomic strategies to gain new insights into the molecular mechanisms of diseases, especially cancer. The topics we are interested about are listed below.

1) To decipher the interactions between biological macromolecules, such as protein-protein interactions and long non-coding RNA (lncRNA)-protein interactions, by combing affinity purification, biological mass spectrometric analysis and bioinformatics, and also to understand their implications in cancer.

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2) Systematic and comprehensive molecular profiling of tumor by multi-omics, integrating data from genetics, transcriptomics, proteomics, metabolomics, etc.

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3) Single cell protein analysis. We employ a state-of-the-art technology called mass cytometry to analyze protein expressions in single cells. Multiplex analytical panels are designed to achieve precise sub-clustering of immune cells in the tumor microenvironment and to understand tumor heterogenesis.

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Honors and Awards
Tianjin Young Elite Scientists Sponsorship Program
Tianjin “131” Innovative Talents
Tianjin Youth Talents for Science and Technology
Patents
Highlighted Publications
Wang H#, Chen L#, Qi L, Jiang N, Zhang Z, Guo H, Song T, Li J, Li H, Zhang N*, Chen R*. A single-cell atlas of tumor-infiltrating immune cells in pancreatic ductal adenocarcinoma. Mol Cell Proteomics. 2022 Jun 17;21(8):100258.
Wang H, Shi X, Gao Y, Zhang X, Zhao H, Wang L, Zhang X, Chen R*. Polystyrene nanoplastics induce profound metabolic shift in human cells as revealed by integrated proteomic and metabolomic analysis. Environ Int. 2022 Jun 15;166:107349
Xu J, Guan X, Jia X, Li H, Chen R*, Lu Y*. In-depth profiling and quantification of the lysine acetylome in hepatocellular carcinoma with a trapped ion mobility mass spectrometer. Mol Cell Proteomics. 2022 Jun 8;21(8):100255.
Yang L, Wang H, Lu W, Yang G, Lin Z, Chen R*, Li H*. Quantitative proteomic analysis of oxaliplatin induced peripheral neurotoxicity. J Proteomics. 2022 Jul 10;266:104682.
Wang Z, Ma W, Wei J, Lan K, Yan S, Chen R*, Qin G*. High-performance olfactory receptor-derived peptide sensor for trimethylamine detection based on Steglich esterification reaction and native chemical ligation connection. Biosens Bioelectron. 2022;195:113673.
Jiang N#, Gao Y#, Xu J, Luo F, Zhang X, Chen R*. A data-independent acquisition (DIA)-based quantification workflow for proteome analysis of 5000 cells. J Pharm Biomed Anal. 2022;216:114795.
Wei J#, Zhao Z#, Lan K, Wang Z, Qin G*, Chen R*. Highly sensitive detection of multiple proteins from single cells by MoS2-FET biosensors. Talanta. 2022; 236:122839.
Wei J, Zhao Z, Luo F, Lan K, Chen R*, Qin G*. Sensitive and quantitative detection of SARS-CoV-2 antibodies from vaccinated serum by MoS2-field effect transistor. 2D Mater. 2022;9:015030.
Zhao D#, Wang C#, Yan S, Chen R*. Advances in the identification of long non-coding RNA binding proteins. Anal Biochem. 2021;639:114520.
Wang H, Zhang Y, Guan X, Li X, Zhao Z, Gao Y, Zhang X, Chen R*. An integrated transcriptomics and proteomics analysis implicates lncRNA MALAT1 in the regulation of lipid metabolism. Mol Cell Proteomics. 2021;100141.
Yan S, Zhao D, Wang C, Wang H, Guan X, Gao Y, Zhang X, Zhang N*, Chen R*. Characterization of RNA-binding proteins in the cell nucleus and cytoplasm. Anal Chim Acta. 2021;1168:338609.
Wang C, Li Y, Yan S, Wang H, Shao X, Xiao M, Yang B, Qin G, Kong R, Chen R*, Zhang N*. Interactome Analysis Reveals that LncRNA HULC Promotes Aerobic Glycolysis through LDHA and PKM2. Nat Commun. 2020;11(1):3162.
Dang Y, Jiang N, Wang H, Chen X, Gao Y, Zhang X, Qin G, Li Y*, Chen R*. Proto-oncogene serine/threonine kinase PIM3 promotes cell migration via modulating Rho GTPase signaling. J Proteome Res. 2020;19(3):1298-1309.
Wang H, Jiang N, Zhang Y, Chen R*. Clinical proteomics: a driving force for cancer therapeutic target discovery and precision medicine. Cancer Biol Med. 2019;16(4):623-629.
Li Y, Zhuang H, Zhang X,Li Y, Liu Y,Yi X, Qin G, Wei W*, Chen R*. Multi-omics integration reveals the landscape of pro-metastasis metabolism in hepatocellular carcinoma. Mol Cell Proteomics. 2018; 17(4):607-618.
Wang H, Shao X, He Q, Wang C, Xia L, Yue D, Qin G, Jia C, Chen R*. Quantitative Proteomics Implicates Rictor/mTORC2 in Cell Adhesion. J Proteome Res. 2018 ;17(10):3360-3369
Chen R, Liu Y, Zhuang H, Yang B, Hei K, Xiao M, Hou C, Gao H, Zhang X, Jia C, Li L, Li Y*, Zhang N*. Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation. Nucleic Acids Res. 2017; 45(17):9947-9959.
Qin G, Dang M, Gao H, Wang H, Luo F, Chen R*. Deciphering the protein-protein interaction network regulating hepatocellular carcinoma metastasis. Biochim Biophys Acta. 2017; 1865(9):1114-1122.
Chen R*, Xiao M, Gao H, Chen Y, Li Y, Liu Y, Zhang N*. Identification of a novel mitochondrial interacting protein of C1QBP using subcellular fractionation coupled with CoIP-MS. Anal Bioanal Chem. 2016; 408(6):1557-64.
Wang Y, Yue D, Xiao M, Qi C, Chen Y, Sun D, Zhang N*, Chen R*.C1QBP negatively regulates the activation of oncoprotein YBX1 in the renal cell carcinoma as revealed by interactomics analysis. J Proteome Res. 2015; 14(2): 804-13.
Gao H, Chen Y, Zuo D, Xiao M, Li Y, Guo H, Zhang N, Chen R*. Quantitative proteomic analysis for high-throughput screening of differential glycoproteins in hepatocellular carcinoma serum. Cancer Biol Med. 2015; 12(3):246-54.
Chen R, Xiao M, Buchberger A, Li L*. Quantitative neuropeptidomics study of the effects of temperature change in the crab Cancer borealis. J Proteome Res. 2014; 13(12):5767-76.
Chen R*, Wang Y, Liu Y, Zhang Q, Zhang X, Zhang F, Shieh CH, Yang D, Zhang N*.Quantitative study of the interactome of PKCζ involved in the EGF-induced tumor cell chemotaxis. J Proteome Res. 2013; 12(3):1478-86.
Zhang X, Zhang F, Guo L, Wang Y, Zhang P, Wang R, Zhang N*, Chen R*. Interactome analysis reveals that C1QBP (complement component 1, q subcomponent binding protein) is associated with cancer cell chemotaxis and metastasis. Mol Cell Proteomics. 2013; 12(11):3199-209.